Gene

Primer sequences, annealing temperatures and lengths of the genes studied

Primer Sense

Primer Anti-sense

Annealing temperature (˚C)

PCR fragment lengths (bp)

gp91^phox

GTGTGTGAATGCCAGAGTCGG

TTCCTGTGATGCCAGCCAAC

54

164

p47^phox

CACCTTCATTCGCCACATCGC

ACGCTGCCCATCATACCACCTG

58

247

p22^phox

CAGAAGTACCTGACCGCTGTGG

GGTAGATCACACTGGCAATGGC

58

157

Rac2

TCAAGTGTGTGGTGGTGGGTG

TGTCTGTGGGTAGGAGAGTGGC

58

215

β2M

CTCAGTTCCACCCACCTCAG

GCAAGCATATACATCGGTCTCG

56

82